|
KMID : 0364820160520040398
|
|
Korean Journal of Microbiology
2016 Volume.52 No. 4 p.398 ~ p.405
|
|
|
Thermoresistant properties of bacterioferritin comigratory protein against high temperature stress in Schizosaccharomyces pombe
|
|
Ryu In-Wang
Lee Su-Hee
Lim Hye-won
Ahn Ki-Sup
Park Kwang-Hark
Sa Jae-Hoon
Jeong Kyung-Jin
Lim Chang-Jin
Kim Kyung-Hoon
|
|
|
Abstract
|
|
|
|
The Schizosaccharomyces pombe structural gene encoding bacterioferritin comigratory protein (BCP) was previously cloned using the shuttle vector pRS316 to generate the BCP-overexpressing plasmid pBCP10. The present work aimed to evaluate the thermoresistant properties of BCP against high temperature stress using the plasmid pBCP10. When the S. pombe cells were grown to the early exponential phase and shifted from 30°C to 37°C or 42°C, the S. pombe cells harboring pBCP10 grew significantly more at both 37°C and 42°C than the vector control cells. After 6 h of the shifting to higher incubation temperatures, they contained the lower reactive oxygen species (ROS) and nitrite content, an index of nitric oxide (NO), than the vector control cells. After the temperature shifts, total glutathione (GSH) content and total superoxide dismutase (SOD) activities were much higher in the S. pombe cells harboring pBCP10 than in the corresponding vector control cells. Taken together, the S. pombe BCP plays a thermoresistant role which might be based upon its ability both to down-regulate ROS and NO levels and to up-regulate antioxidant components, such as total GSH and SOD, and subsequently to maintain thermal stability.
|
|
|
KEYWORD
|
|
|
Schizosaccharomyces pombe, bacterioferritin comigratory protein, glutathione, reactive oxygen species, thermal stress
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|